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Molecular differences between two Jeryl Lynn mumps virus vaccine component strains, JL5 and JL2

机译：两种Jeryl Lynn流行性腮腺炎病毒疫苗组分菌株JL5和JL2之间的分子差异

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The Jeryl Lynn (JL) vaccine against mumps virus (MuV) contains two components, MuVJL5 and MuVJL2, which differ by over 400 nt. Due to the occurrence of bias in the direction of mutation, these differences and those found in nucleotide sequences of different isolates of the minor component in the vaccine (MuVJL2) might be due to the effect of ADAR-like deaminases on MuV grown in tissue-cultured cells. A molecular clone of MuVJL2 (pMuVJL2) and MuVJL2-specific helper plasmids were constructed in order to investigate molecular interactions between MuVJL5 and MuVJL2, to augment the existing molecular clone of MuVJL5 (pMuVJL5) and MuVJL5-specific helper plasmids. Genome and mRNA termini of MuVJL2 were characterized, and an unusual oligo-G insertion transcriptional editing event was detected near the F mRNA polyadenylation site of MuVJL2, but not of MuVJL5. Genes encoding glycoproteins of rMuVJL2 and rMuVJL5 have been exchanged to characterize the oligo-G insertion, which associated with the specific sequence of the F gene of MuVJL2 and not with any other genes or the RNA-dependent RNA polymerase of strain MuVJL2. The results indicate that a single G-to-A sequence change obliterates the co-transcriptional editing of the F mRNA and that this oligo-G insertion does not affect the growth of the virus.
机译：针对腮腺炎病毒（MuV）的Jeryl Lynn（JL）疫苗包含两个成分MuVJL5和MuVJL2，两者相差超过400 nt。由于在突变方向上出现了偏见，这些差异以及在疫苗次要成分不同分离株（MuVJL2）的核苷酸序列中发现的差异，可能是由于ADAR样脱氨酶对组织中生长的MuV的影响所致。培养的细胞。为了研究MuVJL5和MuVJL2之间的分子相互作用，构建了MuVJL2（pMuVJL2）和MuVJL2特异性辅助质粒的分子克隆，以增强MuVJL5（pMuVJL5）和MuVJL5特异性辅助质粒的现有分子克隆。表征了MuVJL2的基因组和mRNA末端，并在MuVJL2的F mRNA多聚腺苷酸化位点附近检测到异常的寡-G插入转录编辑事件，但未检测到MuVJL5。已经交换了编码rMuVJL2和rMuVJL5糖蛋白的基因，以表征寡聚G插入，该插入与MuVJL2 F基因的特定序列相关，而不与任何其他基因或菌株MuVJL2的RNA依赖性RNA聚合酶相关。结果表明，单一的G-to-A序列变化消除了F mRNA的共转录编辑，并且这种寡G插入不会影响病毒的生长。

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